CRC 1118 Diabetes-specific biobank

As a central facility, the CRC 1118 tissue bank secures the availability of scarce Diabetes-related tissue samples and enables their economical and project-specific use.

Tissue samples

The CRC 1118 tissue bank, an intrinsic part of the tissue bank of the tissue bank of the National Center for Tumor Diseases (NCT), processes and archives tissue samples.

The NCT tissue bank has a structural quality management sytem implented according to the DIN EN ISO/IEC 17020 qualifications and holds this accreditation since 2009. To answer specific scientific questions, the CRC 1118 tissue bank is establishing project-specific collections, especially from kidney and nerve tissue. The anatomy of those tissue samples is rated by a qualified pathologist to be able to inform the researchers about disease-related morphological changes in the tissue.

Due to the used technology and methodology, the tissue bank prepares standardised samples (e.g. preparation of tissue slices) and extractions (e.g. DNA) from human tissue. Next to immunohistological stainings (ICH), also speciality stainings such as trichrome staining or PAS staining can be prepared. To facilitate an effortless computer-based workflow, stained slices can be scanned and digitized through virtual microscopy (VM) with the Hamamatsu Nanozoomer.

Diabetes-specific human Tissue-Micro-Arrays (TMAs) have been developed especially for the SFB. The TMAs allow for a increased high throughput in the research with FFPE tissue.  

At the moment there are TMAs from diabetic kidney and nerve samples available. Additional tot he human tissue, several TMAs from experimental diabetes animal models a.o. STZ-mice, Db/Db mice and Ins2Akita mice have been developped in collaboration with A04 and C03. Those TMAs are available for use for all CRC members.


Liquid biosamples

Liquid samples are collected, processed and stored in the Clinic for Endocrinology, Diabetology, Metabolism and Clinical Chemistry.
The collected samples include whole blood, white and red blood cells, serum, plasma as well as urine from diabetics, prediabetics and healthy controls and are stored at -80°C.

An overview of the work at the CRC 1118 tissue bank can be seen in the video at the bottom of this page.

Request forms for tissue samples and services

Please, list the desired tissue samples and services or technologies required in the request form. It is possible to select a combination of multiple options.
Please complete the form on the computer and send it with signatures to Dr. Koenraad Philippaert (contact details on the top right of this site). Please attach a short project description to the request. We are open to discuss any requests concerning tissue availability or required services and provide advice to your project.

There is currently no request form available for liquid samples from the CRC 1118 tissue bank . Please contact us directly if you are interested in those samples.

Please be aware that the use of the diabetes-associated tissue samples are primary intended for the members of the CRC 1118. We can make biomaterial available to external research projects in the context of a collaboration. Please contact us if you are interested.

Dr. Koenraad Philippaert
Institute of Pathology
Biobank CRC 1118
Heidelberg University Hospital
Im Neuenheimer Feld 224
69120 Heidelberg
Germany
Tel:    +49 6221 56 34398
Email

 

Dr. med. Constantin Schwab
Institute of Pathology
Biobank CRC 1118
Heidelberg University Hospital
Im Neuenheimer Feld 224
69120 Heidelberg
Germany

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